Cell culture solutions you can trust
Lonza delivers. Consult the cell culture experts and harness the power of Lonza's extensive experience in delivering bulk cell production & cell isolation services. Just select the cell types and format and we will do the rest. Delays in obtaining vital cell cultures can be very costly. For this reason, we invite you to discover our superior cell culture capabilities, delivered on-time and to your specifications.
Our services include:
Cell line bulk production
At your request, Lonza's cell production facility can produce
billions of cells on-time, every time. By leaving the large scale
production – passaging, feeding, etc. – to the experts at Lonza,
you or your laboratory staff can focus on your experiments
instead of cell provision.
Send us your cell line or alternatively, we can source it for you. At the completion of the project, you will receive your cells, quality tested, and in the format you request.
Primary cell expansion
Do the additional complexities involved in primary cell culture keep you from moving to a more biologically relevant cell model? Are you looking for a provider who has the expertise and proven processes needed to provide you with assay ready primary cells? Lonza, the provider of the Clonetics® and Poietics® brands you trust, is ready to help. Tell us your specifications and we'll deliver the cells and optimized media with the characterization appropriate for your research interests.
Primary cell isolations
Save time and money by avoiding the aggravation of tissue acquisition, failed isolations and low yields. Lonza has decades of experience isolating dozens of cell types from human and animal tissues. We also provide donor matched cell sets and a wide array of QC and cell characterization services. A few examples of successful isolations include:
- Bovine and Porcine preadipocytes
- Canine umbilical vein endothelial cells
- Feline iris smooth muscle cells
- Guinea pig kidney cells
- Human Cystic Fibrosis bronchial/tracheal epithelial cells
- Human small intestine disassociated cells
- Murine dermal fibroblasts
- Porcine mesangial cells
- Rat mesenchymal stem cells
Cells delivered in a variety of formats
Cryopreserved or proliferating formats
- Plates: 6, 12, 24, 48 & 96 well
- Flasks: T-12.5, T-25, T-75, T-150 & T-225
- 1 to 4 ml cryopreserved amps
If what you want isn't on the list, tell us!
Media production
Lonza media products are well-known in the research community for their high quality and consistent performance. We offer classical media, plus an extensive array of serum-free media including protein-free, chemically defined, and non-animal derived formulas. Now the Clonetics ®, Poietics ®, and BioWhittaker ® brands are available in custom basal volumes as well as custom SingleQuots ® volumes & configurations.
Please contact your sales representative or cellsondemand@lonza.com for further information.
Pre-plated conditionally immortalized cells
Conditionally Immortalized Human Myoblasts
- Fig. 4. Differentiation of XM15B1. XM15B1 cells at different passages were differentiated for 7days in differentiation medium (upper panel).
Two different cell lines were developed for human skeletal muscle cells. XM13A1 cells were immortalized using tsSV40LTag, and represent a mixed population of myoblasts isolated from a non-diabetic, 31-40 year old female. Cells grow for more then 50 population doublings with average proliferation rate of 60 hours /PD at 33°C. Cells form large myotubes after 7-8 days of differentiation at 37°C. XM15B1 cells represent a mixed population of myoblasts isolated from healthy male 31-40 years old and immortalized using combination of tsSV40 LTag and hTERT. Cells grow for more then 80 population doublings with average proliferation rate of 40 hours /PD at 33°C, and form large myotubes after 8-10 days of differentiation at 37°C (Fig. 4). Myotubes derived from each cell line express markers typical of differentiation such as desmin, myogenin, GLUT4, UCP 3 and Glycogen Synthase. Functional activities found in conditionally immortalized human skeletal muscle cells include insulin-stimulated glycogen synthesis and insulin-stimulated glucose uptake. Genome-wide comparative expression analysis was performed for primary human skeletal muscle cells and XM15B1. Results show very high degree of correlation between primary and immortalized cells for both myoblasts and myotubes.
Conditionally Immortalized Human Preadipocytes
- Fig.5. Morphology of differentiated XA15A1 cells and primary human adipocytes.
Primary human preadipocytes were isolated from subcutaneous adipose tissue of a non-diabetic, 30-40 year old male donor , and immortalized using tsSV40 LTag. The resulting XA15A1 cells grow for more then 50 population doublings with an average proliferation rate of 40 hours /PD at 33°C. More then 90% of the cells differentiate into adipocytes after 10-12 days of incubation in glitazone-free differentiation buffer at 37°C (Fig. 5). Differentiated cells express both aP2 and Glut4 and demonstrate increased glycerol-3-phosphate dehydrogenase activity. During differentiation, immortalized adipocytes acquire insulin-stimulated fatty acid uptake, glucose uptake and glycogen synthesis, and gain the ability to produce leptin and adiponectin. Genome wide comparative expression analysis was performed for primary human adipocytes and XA15A1. Results show very high degree of correlation between primary and immortalized cells for both proliferating and differentiated cells.
Immortalized human Blood (BEC) and Lymphatic (LEC) Microvascular endothelial cells (donor matched)
Primary BEC and LEC were isolated from the same tissue sample and immortalized using hTERT. The resulting cloned cell lines XSEB113C1 (BEC) and XSEL06C1 (LEC) consist of healthy, homogeneous cells with typical endothelial phenotype. In EGM2-MV medium, XSEB113C1 cells exhibit an average proliferation rate of ~40hrs/PD at 37° C. Cells are >99% CD31 positive/ Podoplanin negative, 80% Factor VIII positive and 100% positive for their ability to uptake Acetylated LDL. These cells maintain responsiveness to VEGF and are able to form tubular structures on MatrigelTM for more than 40PD in culture (Fig.1a). XSEL06C1 cells grow at least 80PD with average proliferation rate of ~30hrs/PD at 37° C. Cells are >99% CD31 positive/ Podoplanin positive, 80% Factor VIII positive and 100% positive for their ability to uptake Acetylated LDL. These cells are responsive to VEGF and form tubular structures on MatrigelTM after more then 60PD in culture (Fig.1b).
Conditionally immortalized human adult Dermal Keratinocytes.
Primary adult keratinocytes were isolated from a healthy, 38 year old Caucasian female donor and immortalized using combination of tsSV40 LTag and hTERT. Two cell lines were developed: a mixed cell population XSKA9B1 and cloned cell line XSKA1B1. XSKA9B1 cells have been cultured continuously for more then 1 year (more than 150PD). Though the cells are heterogeneous at early passages, the phenotype improves after ~ 20PD, and the proliferation rate increases from 66hrs/PD to 36hrs/PD after PD 40 at 33° C. XSKA1B1 (Fig. 2a) was subcloned from XSKA9B1. Cells were cultured for more then 120PD at 33° C with a proliferation rate of ~36hrs/PD. Both cell lines become irreversibly growth arrested at 37 C. Similar to primary keratinocytes, both cell lines are positive for Cytokeratins 1/10, 5, 6, 8, 17, and 19, form tight junctions (as determined by ZO-1 immunostaining and TEER), and maintain the ability to differentiate and stratify in the Epidermal model for more than >50PD in culture (Fig. 2b). Normal karyotype is maintained during passaging.
Immortalized human dermal fibroblasts.
Primary dermal fibroblasts were immortalized using hTERT. The resulting cell line, NDFhTERT, has been uninterruptedly cultured for more than 170PD while maintaining normal phenotype and actively proliferating with the rate of ~40hrs/PD at 37° C. These cells are positive for specific membrane antigens CD90 [(AS02 antibody), Fig. 3a] and Fibroblast MoA (D7-FIB antibody). In addition, the expression of more than 130 genes encoding cell surface proteins was monitored during proliferation and found to be similar to primary human dermal fibroblasts.
Immortalized human Coronary Artery Smooth Muscle cells (CASMC).
Primary human CASMC were immortalized using hTERT. The resulting cell line CASMChTERT has been cultured for over 170PD at 37° C with a proliferation rate of ~30hrs/PD. Cells are 100% positive for alpha-Smooth Muscle Actin (Fig. 3) and Fibroblast MoA (D7-FIB antibody) and are negative for factor VIII and CD90 (AS2 antibody). Cells maintain normal phenotype and karyotype during proliferation. In addition, the expression of more than 130 genes encoding cell surface proteins was monitored during the proliferation and found to be similar to corresponding primary human coronary artery smooth muscle cells.
