Transient Transfection

Transient Transfection – also in primary cells

Research deadlines sometimes do not allow for the generation of a stable clone.  Moreover, in some cases, transiently transfected cells offer advantages over stable transfection. Lonza offers cryopreserved pools of transiently transfected primary cells and cell lines tailored to your research needs.

You will receive:

  • Your transiently transfected primary cells or cell line
  • Detailed information about the transfection procedure
  • Data sheet including transfection efficiency and viability
HUVEC (Lonza CC-2517) were transiently transfected by Nucleofection® with a cAMP inducible luciferase reporter construct (pGL4.29, Promega) and subsequently treated with different Forskolin concentrations.
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