Applications of the Nucleofector^® Technology

The Nucleofector^® Technology is well suited to address specific questions in particular research areas, but is also an ideal platform for applications (such as RNAi-mediated gene silencing and stable transfections) that transcend such divisions.

RNAi is a powerful tool for gene silencing in a highly specific and efficient manner. Efficient down-regulation of gene expression by Nucleofection^® of siRNAs has already been published for many cell types (Citation Database). Using the Nucleofector^® Technology for RNAi is a simple process as the optimal conditions for transfection of siRNA are identical to those for DNA (i.e. as given in our Optimized Protocol for your cell type of choice).

Nucleofection^® has also been successfully tested for protein production. Protein amounts up to 5 mg can be produced transiently within just a few days. The Nucleofector^®  Technology represents the ideal tool for gene transfer even into difficult-to-transfect cell lines like suspension CHO cells.

Nucleofection^® is not only very efficient for transient transfection, it can also be used to generate stably transfected clones especially from difficult-to-transfect cell lines. Such transfectants open new research possibilities including the functional analysis of clones generated from single-integration events and protein production in stably transfected cell lines.

You can also contact our Scientific Support Team to learn more about recent advances in Nucleofector^®  applications.