Delivery of Peptides, Antibodies, Morpholinos or Other Biomolecules
Nucleofector® Technology is not limited to delivery of nucleic acids – it also enables the highly efficient delivery of a diversity of other biomolecules into living mammalian cells.
Use Nucleofector® Technology for non-nucleic acid substrate delivery for applications such as:
- Delivery of peptides and peptide nucleic acids (PNA) for in-vivo interaction studies of peptides and cellular signalling molecules
- Transfer of antibodies into living mammalian cells for the functional study of endogenous factors that are complexed by these antibodies in their natural environment
- Morpholinos (synthetic molecules with nucleic acid-like structure) as highly specific gene knockdown tool especially in animal embryonic systems
- Labelling of cell lines e.g. with X-ray contrast agents for monitoring of in vivo distribution of cells in organism

Delivery of peptides into human neuroblastoma cells. Fluorescence and transmitted light images of SH-SY5Y cells incubated with 50 µM Alexa 488-labelled peptide (dermorphin) with (A, B) and without (C, D) Nucleofection®. (Mollerau C and Roumy M (2006) amaxa news 8: 17-18)

