Nucleofection™ of siRNA Oligos

Efficient Delivery and Knockdown by Nucleofection® of siRNA Duplexes

As proven by more than 300 publications the Nucleofector®  Technology enables researchers to perform succesful siRNA experiments in primary cells and difficult-to-transfect cell lines such as suspension cells. Depending on cell type and target, efficient knockdown can be observed at siRNA concentrations lower than 10 nM.

  • siRNA delivery with up to 99% efficiency
  • Low cytotoxicity
  • Co-transfection with plasmid DNA for transfection control or rescue experiments
  • siRNA library screening in difficult-to-transfect cell types using the 96-well Shuttle®

Nucleofection® outperforms lipofection for effective GAPDH mRNA knockdown in difficult-to-transfect cell types.
Cells were transfected with 5 pmol SMARTpool reagent (Dharmacon) targeting GAPDH using the 96-well Shuttle® (according to the respective optimized protocol) or Reagent L (after titration of optimal reagent amount).  GAPDH mRNA levels were analyzed 24 hours post-transfection by the QuantiGene branched-DNA assay (Panomics) and normalized to siCONTROL Non-Targeting siRNA #1 (Dharmacon).

Data generated in collaboration with Dharmacon, Inc..

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